![]() This discovery made it possible to record the currents of single ion channel molecules for the first time, which improved understanding of the involvement of channels in fundamental cell processes such as action potentials and nerve activity. In this case, the current passing across the membrane is controlled by the experimenter and the resulting changes in voltage are recorded, generally in the form of action potentials.Įrwin Neher and Bert Sakmann developed the patch clamp in the late 1970s and early 1980s. ![]() Alternatively, the current clamp technique can be used. In this case, the voltage across the cell membrane is controlled by the experimenter and the resulting currents are recorded. Patch clamping can be performed using the voltage clamp technique. The technique is especially useful in the study of excitable cells such as neurons, cardiomyocytes, muscle fibers, and pancreatic beta cells, and can also be applied to the study of bacterial ion channels in specially prepared giant spheroplasts. ![]() The patch clamp technique is a laboratory technique in electrophysiology used to study ionic currents in individual isolated living cells, tissue sections, or patches of cell membrane. A patch clamp recording of current reveals transitions between two conductance states of a single ion channel: closed (at top) and open (at bottom).
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